The Journal of Biological Chemistry, Bindi 239

2-mercaptoethanol acetate acid-soluble added addition adenine adenosine albumin aliquot amino ammonium sulfate amount arginine assay ATPase Biochem Biol Biophys brain catalyzed cells centrifugation Chem chromatography coli column compounds concentration containing deoxythymidine described dihydrofolate dihydropteroic acid DNase DPNH effect eluted enzymatic enzyme ester ethanol exonuclease experiments extract fatty acids fluorocitrate folate folic acid formation fraction globin glucose glucose-6-P hexokinase homogenate hydrolysis incorporation increase incubation indicated inhibition inhibitor insulin kinase labeled lactic dehydrogenase lipid liver medium metabolism methyl Mg++ MgCl2 minutes mole mumoles nucleotides observed obtained oxidation perfused phosphatase phosphate buffer phosphorylase polymerase potassium phosphate precipitate prepared present primer procedure protein pteridine purified pyruvate radioactivity ratio reaction mixture samples sodium solution specific activity studies substrate succinic supernatant synthesis Table tetrahydrofolate tion tissue titration triglycerides triphosphates Tris buffer tube tyrosine values volume µmoles мм