The Journal of Biological Chemistry, Bindi 240,Útgáfur 11-12

8-azaguanine acetate Acta added addition adenine albumin aliquot alkaline amino acid ammonium arginine assay Astasia Biochem Biol Biophys carbon cells centrifugation Chem chromatography coenzyme column complex compound concentration containing cyclic cystine cytochrome described determined Dowex EDTA effect eluted enzymatic enzyme ethanol Euglena experiments extract formation fraction galactose glucose glutamate guanine hexokinase histidine hydrolysis inactivation incorporation incubation indicated inhibition isolated kinetics labeled lactic dehydrogenases liver lysine method mg of protein Mg++ mitochondria mole molecular weight molecule nucleotide obtained orotic acid oxidation paper peak peptide peroxidase phosphate plasmin plasminogen prepared present procedure protein purified purines pyridine radioactivity reaction mixture reduced residues sample Sephadex serine serine-P sodium solution solvent specific activity streptokinase studies substrate subunits sulfate supernatant synthesis Table temperature thiol tion tissue titration transfer RNA tryptophan urocanate values volume yeast µmoles мм